Current Issue : July - September Volume : 2016 Issue Number : 3 Articles : 4 Articles
Atopic dermatitis is a skin disease characterized by inflammation, pruritus, and chronic or relapsing\neczematous lesions. Recently, ampholytic polysaccharide sacran has attracted a particular\nfocus of attention as a novel biomaterial. In the present study, we investigated the effect of sacran\nsolution on atopic dermatitis in the clinical study. Almost all of the average scores for atopic dermatitis\nsymptoms of each patient treated with sacran solutions were improved. In addition, the\nscores of sleep disorder and itching were also significantly ameliorated by the sacran treatment\nfor 4 weeks, compared with those of initial states. In immatured dermal skin model stimulated\nwith 2,4-dinitrofluorobenzene (DNFB), the sacran treatment markedly down-regulated inflammatory\ncytokine and chemokine mRNA levels such as MCP-1, TNF-, IL-1, and IL-6 mRNAs, compared\nwith that of DNFB alone. Furthermore, a sacran solution significantly suppressed the mRNA\nexpression of TNF- and COX-2 in RAW264.7 cells, a murine macrophage-like cell line, stimulated\nwith phorbol-12-myristate-13-acetate (PMA). In RBL-2H3 cells, a rat basophilic leukemia cell line,\na sacran solution significantly lowered -hexosaminidase release, indicating the suppression of\nallergic response. These results suggest that a sacran solution may have the potential to improve\natopic dermatitis through the impairment of production of inflammatory cytokine and chemokine\nmRNA....
Background: UV radiation induces significant DNA damage in keratinocytes and is a known risk factor for skin\ncarcinogenesis. However, it has been reported previously that repeated and simultaneous exposure to UV and heat\nstress increases the rate of cutaneous tumour formation in mice. Since constant exposure to high temperatures and\nUV are often experienced in the environment, the effects of exposure to UV and heat needs to be clearly addressed\nin human epidermal cells.\nMethods: In this study, we determined the effects of repeated UVB exposure 1 kJ/m2 followed by heat (39 �°C) to\nhuman keratinocytes. Normal human ex vivo skin models and primary keratinocytes (NHEK) were exposed once a\nday to UVB and/or heat stress for four consecutive days. Cells were then assessed for changes in proliferation,\napoptosis and gene expression at 2 days post-exposure, to determine the cumulative and persistent effects of UV\nand/or heat in skin keratinocytes.\nResults: Using ex vivo skin models and primary keratinocytes in vitro, we showed that UVB plus heat treated\nkeratinocytes exhibit persistent DNA damage, as observed with UVB alone. However, we found that apoptosis was\nsignificantly reduced in UVB plus heat treated samples. Immunohistochemical and whole genome transcription\nanalysis showed that multiple UVB plus heat exposures induced inactivation of the p53-mediated stress response.\nFurthermore, we demonstrated that repeated exposure to UV plus heat induced SIRT1 expression and a decrease in\nacetylated p53 in keratinocytes, which is consistent with the significant downregulation of p53-regulated\npro-apoptotic and DNA damage repair genes in these cells.\nConclusion: Our results suggest that UVB-induced p53-mediated cell cycle arrest and apoptosis are reduced in the\npresence of heat stress, leading to increased survival of DNA damaged cells. Thus, exposure to UVB and heat stress\nmay act synergistically to allow survival of damaged cells, which could have implications for initiation skin\ncarcinogenesis....
Background: Nipple adenoma is a very uncommon, benign proliferative process of lactiferous ducts of the nipple.\nClinically, it often presents as a palpable nipple nodule, a visible nipple skin erosive lesion, and/or with discharge\nfrom the surface of the nipple skin, and is primarily seen in middle-aged women. Resultantly, nipple adenoma can\nclinically mimic the presentation of mammary Paget�s disease of the nipple. The purpose of our current case report\nis to present a comprehensive review of the available data on nipple adenoma, as well as provide useful information to\nhealth care providers (including dermatologists, breast health specialists, and other health care providers) who evaluate\npatients with dermatologic conditions of the breast skin for appropriately clinically recognizing, diagnosing, and\ntreating patients with nipple adenoma.\nCase presentation: Fifty-three year old Caucasian female presented with a one year history of erythema and\ninduration of the skin of the inferior aspect of the right nipple/areolar region. Skin punch biopsies showed\nsubareolar duct papillomatosis. The patient elected to undergo complete surgical excision with right central\nbreast resection. Final histopathologic evaluation confirmed nipple adenoma. The patient is doing well\n31 months after her definitive surgical therapy.\nConclusions: Since nipple adenoma represents a benign proliferative process of the nipple, complete surgical\nexcision is curative. However, the coexistence of nipple adenoma and ipsilateral or contralateral breast cancer is\nwell reported in the literature. The potential for a direct causal link or association of nipple adenoma and breast\ncancer cannot be fully excluded....
Many pathologies of skin, especially ageing and cancer, involve modifications in the matrix alignment. Such tissue reorganization\ncould have impact on cell behaviour and/ormore global biological processes. Tissue engineering provides accurate study model by\nmimicking the skin and it allows the construction of versatile tridimensional models using human cells. It also avoids the use of\nanimals, which gave sometimes non translatable results. Among the various techniques existing, the self-assembly method allows\nproduction of a near native skin, free of exogenous material. After cultivating human dermal fibroblasts in the presence of ascorbate\nduring two weeks, a reseeding of these cells takes place after elevation of the resulting stroma on a permeable ring and culture\npursued for another two weeks. This protocol induces a clear realignment of matrix fibres and cells parallel to the horizon. The\nthickness of this stretched reconstructed tissue is reduced compared to the stroma produced by the standard technique. Cell count\nis also reduced. In conclusion, a new, easy, and inexpensive method to produce aligned tissue free of exogenous material could be\nused for fundamental research applications in dermatology....
Loading....